Fluo 3-AM
Data Sheet
For research use only. Not for human use.
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| CAS No. | 121714-22-5 | Cat. No. | BCP09585 |
| Name | Fluo 3-AM | ||
| Synonyms | Fluo-3-Acetoxymethyl ester; | ||
| SMILES | |||
| Chemical Name | |||
| Formula | C48H46Cl2N2O21 | M. Wt | 1057.79 |
| Purity | 98% | Storage | Store at 4-8°C |
| Description | Fluo-3 is a fluorescent calcium indicator commonly used in flow cytometry (FC) and cell-based experiments to detect changes in intracellular calcium levels. Its absorption maximum at 506 nm makes it compatible with excitation at 488 nm by argon-ion laser sources. Fluo-3 provides intense fluorescence upon binding calcium, detected at a maximum emission at 526 nm which can be monitored by FL1 (green, 525 nm band pass) sensors in flow cytometry. Fluo-3-acetoxymethyl ester is an acetoxymethyl (AM) ester of fluo-3. This form, unlike fluo-3, is cell permeable and readily taken up by living cells, whereupon the AM moiety is enzymatically removed to produce fluo-3. | ||
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