SJB2-043

Data Sheet For research use only. Not for human use.

Cat. No. ：BCP11060CAS No. ：63388-44-3Purity：98%

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CAS No.	63388-44-3	Cat. No.	BCP11060
Name	SJB2-043
Synonyms	SJB2 043;SJB2043;
SMILES
Chemical Name
Formula	C17H9NO3	M. Wt	275.26
Purity	98%	Storage	Store at 4-8°C
Description	Cells were treated with SJB2-043 for 24 hrs and cell extracts were incubated with HA-Ub-VS, followed by immunoblotting with anti-USP1 or anti-HA antibodies. Consistent with our previous studies and the most recent studies by Piatkov et al (24, 31, 33), USP1 in the untreated cells was detected on western blot as a doublet; full length USP1 and an auto-cleaved USP1 with only N-terminus. The Ub-USP1 conjugates were generated in the untreated cell lysates and were detected by an increase in molecular weight of USP1. However, the formation of the Ub-USP1 conjugate was inhibited by the addition of SJB2-043. Ub-aldehyde, a potent known nonspecific DUB inhibitor, caused a complete loss of USP1-Ub-VS conjugates in this competition assay. SJB2-043 inhibited the Ub-VS labeling of a limited number of endogenous DUB enzymes. In addition, SJB2-043 inhibited the labeling of USP1 with Ub-VS in a dose dependent manner.